【摘要】 运用斑马鱼基因芯片技术和实时定量RT-PCR方法,从分子水平上研究了双酚A(BPA)对斑马鱼的毒性作用机制.试验将受精后的斑马鱼胚胎在0.5、1.5和4.5 mg/L BPA暴露8 d,基因芯片并经实时定量RT-PCR验证检测出50个特异基因表达上调或下调、并呈显著的剂量-毒性效应(p<0.05).这些差异表达基因的发现证明BPA具有遗传毒性,并且通过它们功能和作用途径的分析,可以为前期工作中观察到的生理毒性和代谢紊乱等机制研究提供支持.更多还原

【Abstract】 The toxicity mechanism of bisphenol A(BPA) to zebrafish(Danio rerio) was studied in the molecular level,by the method of zebrafish microarray and quantitative real-time reverse transcription polymerase chain reaction(qRT-PCR).Zebrafish embryos were exposed to 0.5,1.5 and 4.5 mg/L BPA for 8 days since fertilization.The results from microarray and validated by qRT-PCR showed that,50 specific genes were up-or down-regulated,and dose-responses for them were significant(p<0.05).This study demonstrates the genotoxicity of BPA.Moreover,according to their function and pathway analysis,it could support the mechanisms for the morphological toxicity and metabolize turbulence observed in prophase study.更多还原