【摘要】 目的观测梗阻性黄疸(OJ)大鼠血浆内皮素(ET-1)及肾皮质微循环的变化,探讨OJ肾损伤的机制及丹参的治疗作用。方法将SD大鼠分为假手术组(SO组);胆总管结扎组(BDL组);丹参干预组(SM组),每组分3、7、10d三个时相,每个时相10只。测定ET-1,血尿素氮(BUN),肌酐(Cr)水平。应用WX-9型微循环显微镜观测3组相应时相肾皮质微血管管径的变化。结果BDL组与SO组比较各时相ET-1水平升高(P<0.05),肾皮质微血管收缩(P<0.05),7、10d血BUN,Cr水平升高(P<0.05)。且随胆道梗阻时间延长变化更加明显(P<0.05)。SM组与BDL组比较各时相ET-1水平下降(P<0.05),7、10d肾皮质微血管扩张,10d血BUN,Cr水平下降(P<0.05)。结论血浆ET-1水平升高及肾脏微循环障碍是梗阻性黄疸肾损害的重要原因,丹参注射液对梗阻性黄疸肾损害有保护作用。更多还原

【Abstract】 Objective To observe the changes of renal cortex microcirculation,ET-1 in obstructive jaundice models and study the effect of Salvia Miltiorrhiz(SM). Methods 90 SD rats were randomly divided into three groups:sham operation group(SO),common bile duct ligation group(BDL),Salvia Miltiorrhiz group(SM). The the caliber of renal cortex capillary vessel was measured by the WX-9 microscope,and the levels of ET-1,blood urea nitrogen(BUN),creatinine(Cr)at 3,7,10 days were detected. Results As compared with SO group,the ET-1 levels in group BDL increased at every time point,but the caliber of capillary decreased,the levels of BUN and Cr were significantly increased at the 7 and 10 days( P < 0. 05),there were more significant changes with the time going( P < 0. 05). AS compared with BDL group,the ET-1 levels in group SM decreased,but the caliber of capillary increased at the 7 and 10 days ( P < 0. 05),and the levels of BUN and Cr significantly decreased( P < 0. 05). Conclusion Obstructive jaundice can induce the microcirculation disturbance of renal cortex and increase the ET-1 levels in rats,and Salvia Miltiorrhiz(SM) play an important role in relieving the kidney injury of obstructive jaundice rats.更多还原