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三株O₇₈、O₁₃₇混合血清型鸡大肠埃希菌iss基因的序列分析

Sequnce Analysis of iss Gene in Three Avian Escherichia coli Strains O₇₈ and O₁₃₇

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【Author】 FAN Chen1,2,WANG Yu3,WANG Hui2,WANG Ya-jun1,4,LI Yi-jing1(1.College of Animal Medicine Sciences,Northeast Agricultural University,Harbin 150030,China;2.Agricultural School,Liaocheng University,Liaocheng 252059,Shandong,China;3.Respiratory Medicine,Liaocheng People’s Hospital,Liaocheng 252000,Shandong,China;4.Wildlife College,Northeast Forestry University,Harbin 150040,China)

【机构】东北农业大学动物医学院；聊城大学农学院；山东省聊城市人民医院呼吸内科；东北林业大学野生动物学院；

【摘要】采用1日龄雏鸡对3株O78、O137混合血清型鸡大肠埃希菌的致病性进行了测定,并扩增了iss基因。结果表明,3株O78、O137混合血清型鸡大肠埃希菌均对1日龄雏鸡具有较强的毒力。iss基因在3株O78、O137混合血清型鸡大肠埃希菌中的序列与已知的禽大肠埃希菌iss基因序列的同源性为100%,与人源大肠埃希菌iss基因序列的同源性为90.9%,显示了此基因的保守性。更多还原

【Abstract】 3 avian Escherichia coli strains O78/O137 were studied.The virulence of each avian E.coli strain O78/O137 for 1-day-old chicks was detected,and each isolate was examined for the presence of iss gene using PCR techniques.Results showed that each avian E.coli strain O78 / O137 was strongly virulent.The sequence of iss from each avian E.coli strain O78 / O137 had 100% identity with AF042279 in GenBank and 90.9% identity with the iss gene from the human E.coli isolate.It suggested that iss gene might be highly conserved.更多还原