【摘要】 以甜瓜叶片基因组DNA为模板,PGIP基因保守序列设计引物,PCR扩增到1条全长978 bp的目的片段。该基因包含有1个完整的开放阅读框,没有内含子,编码326个氨基酸,其编码的氨基酸序列中含有一段典型的亮氨酸重复序列。序列比对表明:该基因编码的氨基酸序列与GenBank中的PGIP基因氨基酸序列(AAP41199)同源性为100%;RT-PCR表达分析表明,该基因在甜瓜叶、茎、根、果实中表达,在花中不表达。为植物分子抗病育种提供了1条基因资源。更多还原

【Abstract】 A target fragment with a full length of 978 bp was amplified with genomic DNA of Cucumis melon L leaves as the templates and the conservative sequences of PGIP gene as the primers.This sequence had a full open reading frame encoding the polygalacturonase-inhibiting protein.The total ORF were comprised by 975 bp of deoxynu-cleotide encoding 325 amino acid.A conserved leucine-rich fragmenthad existed in the derived protein sequence.Se-quencing analysis showed that it was 100% identical with the sequences of PGIP gene which had been cloned.RT-PCR analysis showed that the PGIP gene was expressed in roots,stems,leaves and fruits of cucumis melon L.As a re-sult,a gene resource was provided for molecular breeding of plants.更多还原