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一株产脂肽类生物表面活性剂菌株的分离及代谢产物分析

Isolation and Metabolite Analysis of A Strain Producing Lipopeptide Biosurfactant

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【作者】 罗剑波吴卫霞张凡佘跃惠

【Author】 LUO Jian-bo1,WU Wei-xia2,ZHANG Fan3,SHE Yue-hui1 (1.College of Chemistry and Environmental Engineering,Yangtze University,Jingzhou 434023,China;2.Production Engineering Office,Wenmi Oil Production Plant,Xinjiang Tuha Oilfield Company,Shanshan 838202,China;3.Beijing Bioscience Research Center of Science and Technology,Beijing 100085,China)

【机构】 长江大学化学与环境工程学院新疆吐哈油田公司温米采油厂采油工程室北京百奥赛科技研究中心

【摘要】 采用多次富集培养、血平板筛选方法,从新疆克拉玛依油田油水样中分离得到产生物表面活性剂菌株L1。该菌株与已培养的土壤芽孢杆菌(Brevibacillus agri)的16S rDNA序列同源性达到99%;其代谢产物具有降低表面张力的作用,可以将发酵液表面张力从最初的69.56 mN.m-1降到29.36 mN.m-1;菌株代谢产物经薄层层析分析初步鉴定为脂肽类生物表面活性剂,红外光谱定性该生物表面活性剂属于环脂肽类表面活性剂。

【Abstract】 After multiple rounds of enrichment,blood-plate treatment,strain L1 producing lipopeptide biosurfactant was isolated from the oil water sample of the Kelamayi Oilfield.Phylogenetic analysis based on 16S rDNA gene sequence indicated that strain L1 was closest to Brevibacillus agri with 99% similarity.The surface tension of cultured fluid of strain L1 reduced from 69.56 mN·m-1 to 29.36 mN·m-1,and the metabolite was identified preliminarily as lipopeptide biosurfactant by thin layer chromatography and as a cyclic lipopetide biosurfactant by FTIR spectroscopy.

【基金】 国家863计划资助项目(2008AA06Z204)
  • 【文献出处】 化学与生物工程 ,Chemistry & Bioengineering , 编辑部邮箱 ,2010年02期
  • 【分类号】TQ423
  • 【被引频次】15
  • 【下载频次】607
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