【摘要】 对高效提取啤酒酵母中B族维生素进行了研究,结果表明:啤酒酵母破壁提取VB的工艺采用冻融1次、煮沸30min、加0.1mol/LHCl于80℃提取30min破壁效果较佳,按优化液料比4:1进行一次提取VB的浓度为1.48mg/mL,其提取率为5.92mg/g(以湿酵母计),提取效率为58.77%,累积提取4次(用上一次的提取液累积提取下一批等量的酵母)的VB浓度达3.963mg/mL。另外,2次重复提取可基本将酵母中的VB提取出来,提取效率为96.16%。更多还原

【Abstract】 Extraction of B-vitamins of cerevisiae with high efficiency was studied in this paper.The best freeze thawing times boiling time,HCl concentration,extraction temperature,extraction time and solid-liquid ratio were 1,30 min,0.1 mol/L,80 ℃,30 min and 4:1,respectively,under which yield of B-vitamins was 1.48mg/mL or 5.92 mg/g wet cerevisiae with extracting efficiency 58.77%.The concentration of extractied B-vitamins reached 3.963 mg/mL by cumulatively extraction for 4 times.Besides,nearly all B-vitamins can be extracted from cerevisiae by extraction twice under the optimum conditions and the extraction efficiency was of 96.16%.更多还原