【摘要】 目的探讨HBV前-C区G1896A和BCP区A1762T/G1764A变异与肝细胞癌(HCC)的关系及其可能的机制。方法选择于青岛市传染病医院住院的HBV DNA>104拷贝/ml的慢性HBV感染者82例,其中慢性乙型肝炎(CHB)29例,乙型肝炎肝硬化(LC)27例,HBV相关肝细胞癌(HCC)26例,采用实时荧光PCR法检测其HBV前-C区1896位变异和BCP区1762/1764位变异,并采用ELISA法检测血清肿瘤坏死因子α(TNF-α)水平。结果 HCC组和LC组前-C区G1896A和BCP区A1762T/G1764A变异率、血清TNF-α水平均显著高于CHB组,但HCC组和LC组间无显著差异。前-C区G1896A和BCP区A1762T/G1764A变异者血清TNF-α水平均显著高于非变异组。结论 HBV前-C区G1896A和BCP区A1762T/G1764A变异与HCC形成有关,机制是否与HBV变异和TNF-α水平间的因果关系相关,有待进一步研究。更多还原

【Abstract】 Objective To explore the relationship between the hepatocellular carcinoma,the precore G1896A mutation and the basic core promoter A1762T/G1764A double mutations of the hepatitis B virus. Methods Total of 82 patients with chronic HBV infection hospitalized in Qingdao infectious diseases hospital were enrolled in this study,whose serum HBV DNA levels were higher than 104 copies/ml,including 29 cases of chronic hepatitis B,27 cases of liver cirrhosis and 26 cases of hepatocellular carcinoma. The mutations of precore and basic core promoter of HBV were detected by Real-time fluorescent PCR,the serum TNF-α levels were detected by ELISA. Results The mutation rates of the precore G1896A and BCP A1762T/G1764A in LC group and HCC group were markedly higher than that in CHB group,but were not markedly different between LC group and HCC group. The serum TNF-α levels in patients with precore G1896A and BCP A1762T/G1764A were significantly higher than those in non-mutation patients. Conclusions The mutations of HBV precore G1896A and BCP A1762T/G1764A are correlated with the pathogenesis of HCC,whether it is related to serum TNF-α level remains further discussed.更多还原