【摘要】 目的探索海洛因成瘾大鼠海马区蛋白激酶A(cAMP-dependent protein kinase,PKA)、tau蛋白磷酸化以及成对螺旋纤维(PHF)的改变。方法SD大鼠皮下渐增注射海洛因(1mg.kg-1.d-1逐日递增1mg.kg-1.d-1至20.kg-1.d-1维持10d)建立海洛因成瘾大鼠动物模型。在海洛因成瘾大鼠海马区用免疫组织化学方法针对抗体PKA-α(cAMP-dependent protein kinase-α)、PKA-β(cAMP-dependent protein kinase-β)、tau(S396)、PHF-1(paried helical filaments-1)进行显色。结果海洛因成瘾组大鼠脑内PKA-α、PKA-β、tau(S396)、PHF-1(paried helical filaments-1)免疫染色均明显高于对照组。结论海洛因成瘾大白鼠脑内海马部位存在PKA含量升高、tau蛋白396位点异常磷酸化以及PHF形成。更多还原

【Abstract】 Objective To observe the changes of protein kinase A(PKA),tau hyperphosporylation and paried helical filaments-1(PHF-1)in hippocampus of heroin-addicted rats.Methods By applying the method of increasing dosage day by day,a model of heroin-addicted rats was developed.The expressions of PKA-α、PKA-β、tau(S396)、PHF-1 in hippocampus of the heroin-addicted rats were tested by immunohistochemistry method.Results Compared with the control group,the expressions of PKA-α、PKA-β、tau(S396)、PHF-1 in the hippocampus area of the heroin-addicted rats were higher than those of the control group,the difference had statistical significance(P<0.05).Conclusion The PKA content increased,the 396 situs of tau protein existed over phosphorylation and there were PHF formation in hippocampus of the heroin addicted rats.更多还原