【摘要】 目的探讨职业苯接触与尿中反-反式黏糠酸(ttMA)和苯巯基尿酸(SPMA)的相关性,评定两接触标志物作为生物监测指标的适用性。方法对44名制鞋厂接苯工人进行个体苯暴露水平的作业环境监测,采集当日班前与班后尿样,分别用高效液相色谱和液质联谱测定尿中ttMA和SPMA含量。结果个体苯接触浓度为2.57~146.11 mg/m3,几何平均浓度为(27.91±3.29)mg/m3。班后尿中ttMA和SPMA含量均较班前增高,差异有统计学意义(P<0.01),班后ttMA和SPMA与空气苯浓度的相关系数分别为0.905(P<0.01)和0.537(P<0.01),个体苯接触代谢转化为ttMA和SPMA的相对内暴露指数(RIE)随苯接触浓度的增高而下降。结论在中、高浓度的苯接触时,班后尿ttMA与空气苯浓度的相关性优于SPMA。更多还原

【Abstract】 Objective To investigate correlation of occupational benzene exposure with urinary trans,trans-muconic acid(ttMA) and S-phenylmercapturic acid(SPMA),and assess the applicability of biomarkers in bio-monitoring of benzene exposure.Methods Benzene exposure was measured in 44 shoe-makers and air samples were collected with personal samplers.Pre-shift and post-shift urine samples were collected for ttMA determination by HPLC and for SPMA measurement by HPLC-MS.Results The personal benzene exposure ranged from 2.57 mg/m3 to 146.11 mg/m3 with geometric mean value of(27.91±3.29) mg/m3.Both levels of urinary ttMA and SPMA in post-shift were higher than those in pre-shift(P<0.01) and were significantly correlated with personal benzene exposure(Pearson r=0.905,P<0.01 and r=0.537,P<0.01,respectively).The relative internal exposure index(RIE) of benzene bio-transformed metabolites decreased with increasing of the benzene concentration.Conclusions The correlation of post-shift urinary ttMA concentrations with benzene exposure levels is superior to that of SPMA in moderate and high levels of benzene exposure.更多还原