【摘要】 目的:构建肝硬化门脉高压症动物模型,为该模型建立一种新术式应用于临床治疗的可行性提供理论基础和科学依据。方法:①45头健康广西巴马小型猪分为对照组15头,实验组分成A、B两组,每组各15头;②采用CCL4腹腔注射法和口服饲喂法构建肝硬化门脉高压症动物模型。结果:①实验A、B组成模时间分别为70 d和140 d,成模率分别为100%和80%;②动物模型的门脉压力,对照组为(1.96±0.06)kPa,实验A组为(2.90±0.27)kPa,实验B组为(2.90±0.28)kPa,与对照组比较差异有统计学意义(P<0.05)。结论:CCL4腹腔注射法构建的肝硬化门脉高压症动物模型优于口服饲喂法,造模时间短,成模率高,动物死亡率低,是一种理想的造模方法。更多还原

【Abstract】 Objective:To establish cirrhotic animal model with portal hypertension for providing the rationale and science basis for this technique’s application in clinically treating cirrhotic patients with portal hypertension.Methods:①Fourty-five health small-scale pigs(Bama County,Guangxi) were randomly divided into normal control(n=15) and experimental group(n=30).The experimental group was randomly divided into two subgroups equally.②Cirrhotic animal model with portal hypertension was established by CCL4 intraperitoneal injection and oral administration.③All data were analyzed by SPSS 13.0 statistical package.Results:①Group A: The time of success is 10 weeks and the rate of success is 100 percent.Group B: The time of success is 20 weeks and the rate of success is 80 percent.②The portal pressure in normal control group is(1.96±0.06)kPa,the portal pressure in group A is(2.90±0.27)kPa,the portal pressure in group B is(2.90±0.28)kPa.The differences between the two groups were significant(P<0.05).Conclusion:The CCL4intraperitoneal injection method is better than that of the oral administration on establishing cirrhotic animal model with portal hypertension.The CCL4intraperitoneal injection method has a shorter time period and has a higher success rate compared with oral administration on setting up cirrhotic animal model with portal hypertension.更多还原