【摘要】 目的:观察胎盘免疫调节因子(placenta factor,PF)对PC12细胞生长的影响。方法:培养PC12细胞,用MTT法观察PF对体外无血清培养PC12细胞存活率的影响及PF对低血清培养PC12细胞增殖、分化的影响。结果:对无血清培养的PC12细胞加入不同浓度的PF培养44h后,PF在浓度为50、25、12.5、6.25mg/L时可以显著提高无血清培养PC12细胞的存活率(P<0.05)。对低血清培养的PC12细胞加入不同浓度的PF培养68h后,PF在浓度为12.5mg/L和6.25mg/L时可以显著提高PC12细胞的数量(P<0.01)。对低血清培养的PC12细胞加入不同浓度的PF培养10d,PF在浓度为3.13mg/L和1.56mg/L作用第6天时,细胞长出突起。结论:PF能提高无血清培养PC12细胞的存活率,促进PC12细胞增殖,诱导PC12细胞分化。更多还原

【Abstract】 Objective:To observe the effects of placenta factor on the growth of PC12 cells.Methods:The effect of PF on the survival rate of PC12 cells in serum-free medium and the proliferation and differentiation of PC12 cells in the low serum medium were determined by MTT test.The morphology changes of PC12 cells were observed by ordinary microscopic.Results:At the concentrations of 50 mg/L,25 mg/L,12.5 mg/L and 6.25 mg/L,PF could significantly increase the survival rate of PC12 cells in serum-free medium by different concentrations of PF for PC12 cells 44 hours (P<0.05).At the concentrations of 12.5 mg/L and 6.25 mg/L,PF could significantly increase the number of PC12 cells in the low serum medium by different concentrations of PF for PC12 cells 68 hours(P<0.01).During treating PC12 cells in the low serum medium with different concentrations of PF for 10 days,PC12 cells grew protuberance at the concentration of 3.13 mg/L and 1.56 mg/L of PF when PC12 cells had grown six days.Conclusion:The study showed that PF could enhance the survival rate of PC12 cells in serum-free medium,inhibit the proliferation of PC12 cells and induce the differentiation of PC12 cells.更多还原