【摘要】 以牛尾菜顶芽为外植体,探讨不同浓度6-BA(0.5～5.0 mg/L)和NAA(0.1～1.0 mg/L)配比对丛生芽诱导、增殖和生根的影响。结果表明,适宜丛生芽诱导的培养基为MS+6-BA 2.0 mg/L+NAA 0.2 mg/L+0.3%AC,丛生芽萌动早、无褐化,诱导率为75.0%;丛生芽增殖最佳培养基为MS+6-BA 0.5 mg/L+NAA 0.1 mg/L,增殖系数为4.2;生根最佳培养基为1/2MS+NAA 1.0 mg/L,生根率为87.0%,根较粗壮,生根数4～7条,根平均长度为1.2 cm;在混合基质营养土、木屑、黄泥(1∶1∶2)中,幼苗移栽成活率达80.0%以上。更多还原

【Abstract】 The shoot tip of wild Smilax riparia A.DC was used as explants to conduct experiment on the induction,multiplication and rooting of cluster buds in media with different combinations of 6-BA(0.5-5.0 mg/L) and NAA(0.1-1.0 mg/L).The results showed that the optimum medium for cluster buds induction was MS+6-BA 2.0 mg/L+NAA 0.2 mg/L+ 0.3% activated charcoal.Browning of explants was completely controlled in this medium and the cluster buds grew earlier with 75.0 % induction rate.The optimum medium for multiplication of cluster buds was MS+6-BA 0.5 mg/L+NAA 0.1 mg/ L with 4.2 multiplication coefficient.The optimum medium for rooting was 1/2MS supplemented with 1.0 mg/L NAA,the rooting rate reached up to 87.0%,the number of root was from 4 to 7 and the average root length reached up to 1.2 cm.The rooting plantlets were found able to survive in the transplantation medium(nutrition soil ∶ sawdust ∶ yellow mud= 1∶1∶2) with 80.0% of transplant survival rate.更多还原