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猪圆环病毒2型PCR检测方法的建立及应用

Establishment and Application of PCR Detection Porcine Circovirus Type 2

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【作者】郑玉姝；赵朴；刘俊伟；陈金山；姚四新；赵坤；李任峰；王三虎；刘兴友；张智勇；

【Author】 ZHENG Yu-shu1,2, ZHAO Pu1, LIU Jun-wei1, CHEN Jin-shan1, YAO Si-xin1, ZHAO Kun1, LI Ren-feng1, WANG San-hu1,2, LIU Xing-you1,2, ZHANG Zhi-yong1 (1.Department of Animal Science and Technology,Henan Institute of Science and Technology, Xinxiang 453003, China; 2.Henan Engineering Research Center of Animal Disease and Residue Prevention and Control,xinxiang 453003,China)

【机构】河南科技学院动物科学学院；动物疫病和残留物防控河南省高校工程技术研究中心；

【摘要】根据GenBank登录的PCV2的保守序列,设计1对引物,建立了PCV2的PCR检测方法。试验结果表明,该PCR方法敏感、特异,适于临床样品检测,为PCV2的临床诊断和流行病学调查奠定了基础。更多还原

【Abstract】 According to the conserved sequence of PCV2 published in GenBank, a pair of primers for amplification of PCV2 specified fragment was designed and a PCR assay for PCV2 detection was established. The results showed that the PCR was sensitive and specific, and useful to detect the clinic samples. It was helpful for clinical diagnosis and epidemiologic survey of PCV2.更多还原

【关键词】猪圆环病毒2型； PCR；临床诊断；流行病学调查；
【Key words】 porcine circovirus type 2； PCR； clinical diagnosis； epidemiologic survey；

【文献出处】中国畜牧兽医 ,China Animal Husbandry & Veterinary Medicine , 编辑部邮箱 ,2010年08期

【分类号】S858.28
【被引频次】14
【下载频次】180

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