【摘要】 本试验在成功筛选出抗鸡大肠杆菌(O78)效果较好的中药复方基础上,对该复方进行超微粉制剂的研制,并采用人工感染鸡大肠杆菌病的方法对超微粉与普通粉制剂进行药效比较试验。结果表明:①扫描电镜下,超微粉的形态发生了明显的变化,大多破碎成组织碎片,且大小分布均匀;光学显微镜下,超微粉中的组织细胞均被破坏,出现断裂或形成碎片,绝大多数细胞破壁,而普通粉的组织细胞仍完整(如石细胞、导管等明显存在);在相同的条件下(25℃,浸泡60 min)乌梅超微粉的有效成分枸橼酸的溶出量比普通粉提高了78.6%,超微粉的溶出度增大;②超微粉高、中剂量组的死亡率均极显著低于感染对照组(P<0.01),超微粉低剂量组、普通粉对照组的死亡率略低于感染对照组(P>0.05);超微粉高剂量组死亡率显著低于普通粉对照组(P<0.05),治愈率、有效率显著高于普通粉对照组(P<0.05)。更多还原

【Abstract】 Compound traditional chinese medicine(CTCM) had been selected successfully in the test,which was identified as the best anti-bacterial effect against colibacillosis in chickens.CTCM was conducted to the development of ultrafine powder preparation,as well as the therapeutic effect contrast test of ultra-fine powder and common fine powder of CTCM in chickens artificially infected with colibacillosis.The results showed that:①Observed by scanning electronic microscope and optical microscope.The profiles of different powders were distinctly different between ultra-fine and common fine powder.Comparing with the common fine powder,the particle size of ultra-fine powder was smaller and distributed more regularly.Only fragment of tissue was observed as tissue cell was broken in ultra-fine powder while the tissue cell of common fine powder was still intact and the primary profile could be observed(e.g.stone cell and catheter) in common fine powder.The dissolution test showed that the dissolution of citric acid,main active component of Ebony,was increased by 78.6 percent under the same condition.Comparing the inhibition effect against avian E.coli,the ultra-fine powder through simple extracted showed a better antimicrobial behavior than that extracted from common fine powder of CTCM.②The experiment in which the diseased chicken were treated by feeding with CTCM,indicated that the mortality of the high and middle dose groups of ultra-fine powder was significantly lower than the infected control group(P<0.01),however,the groups of the low ultra-fine powder dose and common fine powder had no significant difference compared to the infected control group(P>0.05);in the high dose group of ultra-fine powder,the mortality rate was significantly lower than the common fine powder group(P<0.05),and the cure and effective rate also showed significantly difference between the two groups(P<0.05).更多还原