【摘要】 目的探讨自身抗体以及体液免疫对ANA阴性系统性红斑狼疮(SLE)的诊断价值和临床意义。方法抗核抗体(ANA)的检测采用间接免疫荧光法;抗ds-DNA抗体的检测采用绿蝇短膜虫为底物的间接免疫荧光法,ENA采用免疫印迹法。免疫球蛋白及补体采用速率散射比浊法。结果 ANA阴性的SLE与其他自身免疫性疾病(AID)比较,抗dsDNA、抗Sm、抗核小体的阳性率有显著差异P<0.05,而体液免疫IGG、IGA、IGM、C3、C4与其他AID比较,差异无统计学意义P>0.05。结论 ANA阴性的SLE,抗dsDNA、抗Sm、抗核小体阳性仍对SLE具有重要诊断价值,免疫球蛋白和补体C3、C4具有参考价值。更多还原

【Abstract】 Objective To determine the diagnosis value and clinical significance of autoantibody and humoralimmunity in SLE patients with negative ANA.Methods ANA was tested by indirect immunofluoresence technique;Anti-dsDNA antibody was examined by erithidia lueiliae immunofluresent technique;Extractable nuclear antigen(ENA) was detected by immunobloting technique;immunoglobins and complements were tested by rate nephelometry technique.Results Compared with other auto immune diseases(AID),the positive rates existed significant difference among anti-dsDNA antibody,anti-SM antibody and anti-nucleosome antibody in SLE patients with negative ANA.while the differences of positive rates did not exist between the two groups among IgG,IgA,IgM,C3 and C4.Conclusion Positive results of anti-dsDNA antibody,anti-SM antibody and anti-nucleosome antibody have great value to SLE patients with negative ANA,while immunoglobins and complements have reference value.更多还原