【摘要】 目的探讨丙型肝炎病毒基因组产物对环加氧酶2(COX-2)表达的影响。方法将带有COX-2基因启动子的报告质粒分别与10种表达丙型肝炎病毒基因组的单个基因的质粒共转染Huh7细胞,并测定荧光色素酶的活性,采用反转录PCR和Western blot检测COX-2的mRNA和蛋白表达的变化,酶联免疫吸附试验检测前列腺素E2含量的改变,探讨这些丙型肝炎基因组产物与COX-2基因启动子之间的相互作用。结果在所有10个基因组产物中,非结构蛋白NS3能够显著地激活COX-2基因启动子的活性,并在mRNA和蛋白水平上调COX-2的表达。而且这种激活作用呈剂量依赖性,前列腺素E2的分泌量随着NS3蛋白表达量的增加而增加。结论 NS3蛋白能功能性地激活COX-2的表达。更多还原

【Abstract】 Objective To investigate the effect of HCV genomic products on the expression of cyclooxygenase-2(COX-2). Methods Huh7 cells were co-transfected with reporter plasmid pCOX-2-Luc containing the luciferase gene under the control of COX-2 promoter and plasmids carrying individual genes of HCV,luciferase activity was measured,expression of COX-2 was measured by RT-PCR and western blot,and prostaglandin E2(PGE2) was measured by enzyme-linked immunosorbent assay(ELISA). Results In these HCV genomic products,Non-structural protein NS3 enhances COX-2 gene promoter activity,COX-2 mRNA expression, COX-2 protein production,and PGE2 release in Huh7 cells in a concentration-dependent fashion.Conclusion NS3 protein activated COX-2 expression was functional.更多还原