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醋酸铅棉球法筛选口腔产硫化氢细菌的研究

Preliminary study of screening oral bacteria producing hydrogen sulfide by cotton balls with lead acetate

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【作者】 孙崇奎卢礼兵朱硃龚其美肖晓蓉肖丽英周学东李燕

【Author】 SUN Chong-kui,LU Li-bing,ZHU Zhu,GONG Qi-mei,XIAO Xiao-rong,XIAO Li-ying,ZHOU Xue-dong,LI Yan. (State Key Laboratory of Oral Diseases,Sichuan University,Chengdu 610041,China)

【机构】 口腔疾病研究国家重点实验室,四川大学

【摘要】 目的对口腔常见细菌的产硫化氢能力进行筛选,期望找到主要的口臭致病菌,分析致臭菌与龋病和牙周病的关系。方法以醋酸铅棉球法间接定性链球菌属、放线菌属、乳杆菌属的8种可疑致龋菌以及卟啉单胞菌属、普雷沃菌属、梭杆菌属等9种牙周可疑致病菌产硫化氢的能力。结果 9种牙周病可疑致病菌均可产生硫化氢。在致龋菌中,格氏链球菌、内氏放线菌、溶牙放线菌、嗜酸乳杆菌产硫化氢,变异链球菌、表兄链球菌、黏性放线菌和发酵乳杆菌不能产生硫化氢。结论产硫化氢的细菌大多数为革兰阴性厌氧菌,致臭菌与牙周病、牙髓病的发生发展存在着相关性。约半数的致龋菌能产生硫化氢,导致口臭的发生。

【Abstract】 Objective To screen common oral bacteria which produce hydrogen sulfide,and expect to find the main source of bad breath. To analyze the relationship between halitosis and oral caries,periodontal diseases. Methods Based on the color reaction between hydrogen sulfide and lead acetate,we use indirect experimental method by cotton-wool to detect 8 bacteria as Streptococcus,Actinomyces,Lactobacillas which are about caries and 9 bacteria as Porphyromonas,Prevotella,Fusobacterium which are about periodontal to identify the ability to produce hydrogen sulfide. Results All of the 9 bacteria about periodontal disease can produce H2S. In the bac-teria group about caries,only Streptococcus gordonii,Actinomyces naeslundii,Actinomyces odontolyticus,Lacto-bacillus acidophilus can produce H2S while Streptococcus mutans,Streptococcus sobrinus,Actinomyces viscosus,Lactobacillus fermentatae can not. Conclusion Most of the bacteria which can produce H2S are gram-negative anaerobic bacteria. The bacteria that produce halitosis have relationship with the occurrence and development of periodontal disease,pulposis. And almost one half of the cariogenic bacteria can produce hydrogen sulfide,which can result halitosis.

【基金】 口腔疾病研究国家重点实验室自主研究基金资助项目(SKLODPI012)
  • 【文献出处】 国际口腔医学杂志 ,International Journal of Stomatology , 编辑部邮箱 ,2010年05期
  • 【分类号】R780.2
  • 【被引频次】2
  • 【下载频次】231
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