【摘要】 目的探讨骨膜对游离移植骨血管生成的影响。方法将36只健康成年新西兰大白兔双侧桡骨部分截断,一侧保留完整骨膜(试验侧),另一侧剥离骨膜(对照侧),分别植入兔的双侧咬肌区,术后3d以及1、2、3、4、6周处死动物取材,通过血管墨汁灌注、CD34免疫组化染色、四环素荧光标志等方法检测移植骨微血管密度和四环素荧光标志积分光密度值,观察移植骨的再血管化和新骨形成情况。结果术后3d至1周,试验侧和对照侧的再血管化程度及新骨形成速度无差异(P>0.05)。两组在术后2周开始出现再血管化和新骨形成;术后2～6周,试验侧的再血管化程度和新骨形成速度高于对照侧(P<0.05)。结论骨膜能使非血管化自身游离移植骨再血管化程度增加,血管生成的增加在一定程度上能使新骨形成加快,从而加速移植骨的愈合。更多还原

【Abstract】 Objective The purpose of the present study is to analyze effect of periosteum in angiogenesis of non-vascularied autogenous bone grafts.Methods Thirty-six New Zealand white rabbits were subjected to this study.Bilateral radial bones were partially removed as free bone grafts,half of which periostea were intact while rest of which periostea were peeled off.All grafts were implanted in the masseter muscle separately.At 3 days,1,2,3,4 and 6 weeks after surgery the rabbits were sacrificed and the specimens were procured for Chinese ink perfusion,CD34 immunohistochemistry stain and tetracycline fluore-scence labeling.Microvessel density(MVD)and tetracycline fluorescence labeling intergrated optical density(D)i were assessed in order to evaluate angiogenesis and rate of new bone formation of autogenous bone grafts.Results At 3 days and 1 week after surgery,MVD and Di of two groups had no significant difference(P>0.05).Revascularization and new bone formation of both groups were found at 2 weeks after surgery.At 2,3,4 and 6 weeks after surgery,MVD and Di of test group were significantly higher than those of control group(P<0.05).Conclusion Angiogenesis in non-vascularized auto-genous bone graft was promoted when periosteum of graft was intact.Thereby followed osteogenesis was also accel-erated.更多还原