【摘要】 利用化学计量学分辨方法和高效液相色谱法建立了黄芪中毛蕊异黄酮和芒柄花素的定量分析方法。色谱柱为Hypersic ODS柱(4.4mm×250mm,5μm),流动相为A:水含0.2%(V/V)醋酸,B:乙腈含0.2%(V/V)醋酸,线性梯度洗脱。借直观推导式演进特征投影(HELP)法分辨HPLC-DAD产生的二维数据得毛蕊异黄酮和芒柄花素的纯光谱和纯色谱,用总体积积分法定量分析其含量。毛蕊异黄酮和芒柄花素的校准曲线和相关系数分别为y=166.72x+127.8(r=0.9997)和y=178.39x+187.2(r=0.9993),线性范围分别为1.0—116.5μg·mL-1和1.2—112.5μg·mL-1,加标回收率分别在96.5%—98.7%和96.8%—98.5%之间。方法灵敏、选择性和可靠性好,可用于测定黄芪中毛蕊异黄酮和芒柄花素含量及质量控制。更多还原

【Abstract】 A method for the determination of calycosin and formononetin in radix astragal was developed with relative chemometrics methods and high performance liquid chromatography.The analytical column was Hypersic ODS column(4.4mm×250mm,5μm).The mobile phase was water containing 0.2%(V/V) acetic acid (A) and acetonitrile containing 0.2% (V/V) acetic acid(B) with a gradient system.The two-way data of HPLC-DAD hyphenated instrument was resolved with heuristic evolving latent projections (HELP) method and the pure chromatogram and spectra of each component were obtained.Then calycosin and formononetin were determined with overall volume integration method.The regression equations and coefficients were y=166.72x+127.8 and 0.9997 for calycosin,y=178.39x+187.2 and 0.9993 for formononetin.The liner ranges were 1.0—116.5μg·mL-1 and 1.2—112.5μg·mL-1 for calycosin and formononetin,respectively.The standard added recoveries were in the range of 96.5%—98.7% and 96.8%—98.5% for calycosin and formononetin,respectively.This method is highly sensitive,selective and reliable,and is used for simultaneous determination of calycosin and formononetin in radix astragal and the quality control of radix astragal.更多还原