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高效液相色谱法测定复方甘草酸苷氯化钠注射液中的甘草酸单铵含量
Determination of the Contents of Glycyrrhizin in Glycyrrhizin Injection by HPLC
【摘要】 高效液相色谱法测定复方甘草酸苷氯化钠注射液中的甘草酸单铵。采用ODS-C18色谱柱;乙腈-2%冰醋酸(40:60)为流动相;流速1.0mL.min-1;检测波长254nm;柱温为室温。该方法检出限为8ng/mL,线性范围为0.05—0.80mg/mL(r=0.9989),平均回收率98.81%。
【Abstract】 The content of glycyrrhizin in glycyrrhizin injection was determined by HPLC with ODS-C18 column,acetonitrile and 2% acetic acid(40∶60)as mobile phase at 254nm,room temperature,and flow rate of 1.0mL/min.Linear range of glycyrrhizin was 0.05—0.80mg/mL(r=0.9989).The average recovery was 98.81%.
- 【文献出处】 光谱实验室 ,Chinese Journal of Spectroscopy Laboratory , 编辑部邮箱 ,2010年02期
- 【分类号】R927.2
- 【下载频次】146