【摘要】 以Eu2O3试剂为荧光前驱体,与钛酸正丁酯在酸-水溶液中发生溶胶-凝胶反应,制备了一种可作为荧光标记分子的Eu3+/TiO2荧光性纳米微粒,并对样品的形貌、组成及结构进行了表征。结果表明,纳米粒子的平均粒径5nm。用468nm激发光源激发样品,荧光强度随着Eu3+的掺杂含量0、0.5%、1%、2%、3%、4%先升高后降低,当掺杂含量为2%时,荧光强度最强。采用倒置荧光显微镜观察了Eu3+/TiO2纳米荧光探针在洋葱表皮细胞膜上聚集受激发射荧光行为。更多还原

【Abstract】 In this work, using Eu2O3 as the fluorescent precursor, the Eu3+/TiO2 nanocrystals are synthesized by the sol-gel method. Morphologies, compositions and structures of the samples are characterized. The results show that the average diameter of particles is about 5nm. The Eu3+contents(molar ratio) in the samples are 0, 0. 5%, 1%, 2%, 3% and 4%. Excitation with a wavelength of 468nm result in photoluminescent emission. The photoluminescence of Eu3+in Eu3+/TiO2 nanocrystals has the strongest emission intensity at 2% of Eu3+ concentration. Fluorescence microscopy is adopted to investigate their emission fluorescence of onion epidermal cells.更多还原