【摘要】 目的建立婴幼儿腹泻颗粒中葛根素检测的质量标准。方法采用薄层色谱法进行定性鉴别,以RP-HPLC测定葛根素的含量。采用C18柱,流动相为乙腈一水(10:90),检测波长为250nm。结果薄层色谱分离度较好,专属性强。葛根素的线性范围为0.482～4.820μg(r=0.99992),平均回收率98.2%。RSD为1.78%。结论:建立的定性、定量方法简便、准确、专属性强,可有效控制婴幼儿腹泻颗粒的质量。更多还原

【Abstract】 Objective:To establish the quality standards of puerarin in infant diarrhea grain.Methods:We made qualitative identification by using the thin-layer chromatography(TLC),determining the puerarin content using RP-HPLC method.The chromatographic assay was performed on a C18column(5μm,4.6 × 250 mm),the mobile phase consisted of a water-acetonitrile(10:90),the detective wavelength was set at 250nm.Results:TLC method has a better separation and specificity.The Linear range of puerarin was from 0.482 to 4.820 μg,and its average recovery rate was 98.2%.RSD was 1.78%.Conclusion:This method is simple,accurate and specificity for the determination of puerarin,which can effectively control the quality of this grain.更多还原