【摘要】 目的建立测定人血浆中兰索拉唑(抗胃及十二指肠溃疡药)含量的超快速液相色谱-质谱/质谱联用法(UFLC-MS/MS)。方法血浆样品经甲醇沉淀蛋白后,以乙腈-0.1%甲酸(27∶73)洗脱,用UltimateXB-CN(5μm,2.1mm×150mm)色谱柱,通过电喷雾离子化三重四级杆串联质谱,经多反应监测模式检测,m/z370.4→m/z252.2(兰索拉唑),m/z237.2→m/z194.3(卡马西平,内标)。结果兰索拉唑在5.01～5007.90μg.L-1内线性关系良好(γ=0.9985),最低定量浓度为5.01μg.L-1。方法回收率(n=5)分别为90.21%,89.71%,92.27%,日内和日间精密度均<9%。结论该法操作简单,灵敏,准确,重现性好,适用于该药的临床药代动力学研究。更多还原

【Abstract】 Objective To establish an UFLC-MS/MS method for the determination of lansoprazole concentration in human plasma.Methods The plasma was deproteinated by methanol.The separation was performed on an Ultimate XB-CN(5 μm,2.1 mm×150 mm)columm.The mobile phase consisted of acetonitrile-water(27∶73)containing 0.1% formic acid was used with the gradient elution.Detection was performed on a tandem mass spectrometry with electrospray ionization sourse in positive ion mode.Multiple reaction monitoring(MRM)mode with the transition of m/z 370.4→m/z 252.2(lansoprazole)and m/z 237.2→m/z 194.3(carbamazepine)was used to quantify lansoprazole and internal standard respectively.Results The linear range was 5.01-5007.90 μg·L-1,and the limit of quantitation was 5.01 μg·L-1.The method recoveries were 90.21%,89.71%,92.27% respectively and the RSD of inter-day and intra-day was less than 9%.Conclusion The method is simple,sensitive,accurate and reproducible.It is applicable for pharmacokinetic study of lansoprazole.更多还原