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NaCl胁迫对花生种子萌发的影响

Effects of NaCl stress on seed germination of peanut

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【作者】 郭峰万书波李新国徐平丽孟静静

【Author】 GUO Feng1,2,WAN Shu-bo1,2,LI Xin-guo1,2,XU Ping-li1,2,MENG Jing-jing1,2(1.High-tech Research Center,Shandong Academy of Agricultural Sciences;Key Laboratory for Genetic Improvement of Crop,Animal and Poultry of Shandong Province,Ji’nan,Shandong 250100,China;2.Key Laboratory of Crop Genetic Improvement and Biotechnology,Huanghuaihai,Ministry of Agriculture,Ji’nan,Shandong 250100,China)

【机构】 山东省农业科学院高新技术研究中心山东省作物与畜禽品种改良生物技术重点实验室农业部黄淮海作物遗传改良与生物技术重点开放实验室

【摘要】 以花生品种鲁花14号(LH14)和丰花1号(FH1)为材料,通过对不同NaCl浓度(0、1002、00、300、4005、00mmol/L)处理的花生种子研究表明:低浓度NaCl处理(100 mmol/L)对花生种子的萌发影响相对较小;随着NaCl浓度增加,花生种子的发芽势和发芽率逐渐降低,根长、根表面积、根体积、根鲜重和胚根粗逐渐减小,MDA和脯氨酸含量逐渐增加;高浓度NaCl处理情况下,花生根尖DNA凝胶电泳出现明显的连续拖带现象,根尖细胞凋亡相对严重。

【Abstract】 Peanut seeds treated with different NaCl concentrations(0,100,200,300,400 and 500 mmol/L) were studied with peanut cultivar Luhua 14(LH14) and Fenghua 1(FH1).The main results were described as follows.The effect of low concentration of NaCl treatment((100 mmol/L) on peanut seed germination was relatively small.With the increase of NaCl concentration,peanut seed germination energy and germination rate decreased gradually,the same as root length,root surface area,root volume,root fresh weight and radicle diameter.But MDA and proline content increased gradually.DNA gel electrophoresis in the peanut root tip with high concentration of NaCl had obvious continuous band, so the apoptosis of root tip cells was relatively serious.

【基金】 农业部现代农业产业技术体系建设专项资金资助(nycytx-19);国家“十一五”科技支撑计划项目“花生优质安全增效关键技术研究与示范”(2006BAD21B04)
  • 【文献出处】 干旱地区农业研究 ,Agricultural Research in the Arid Areas , 编辑部邮箱 ,2010年03期
  • 【分类号】S565.2
  • 【被引频次】55
  • 【下载频次】661
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