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角茎野牡丹快速繁殖的研究
Rapid Propagation of Tibouchina granulosa
【摘要】 以茎段营养芽为材料,进行了角茎野牡丹的快速繁殖研究。结果表明:培养基MS+BA 0.3 mg.L-1+NAA 0.01 mg.L-1最适宜芽增殖,增殖系数达到3.1倍;在培养基1/2MS+NAA0.3 mg.L-1上无根苗培养7 d生根率85.0%,20 d生根率100%,每苗平均根数6.3条。试管苗移植成活率95.0%。
【Abstract】 The rapid propagation of Tibouchina granulosa by using stem segment as materials was studied.The results showed that the optimal medium for inducing adventitious buds was MS+ BA 0.3 mg·L-1+ NAA 0.01 mg·L-1 and the proliferation of multiples was 3.1.After cultured 7 days on the medium of 1/2 MS+NAA0.3 mg·L-1,the rooting rate of shoots was 85.0% while after 20 days the rate reached 100% and the average number of roots was 6.3 The survival rate of the transplanted test-tube plantlets was 95.0%.
【关键词】 角茎野牡丹;
不定芽;
快速繁殖;
组织培养;
【Key words】 Tibouchina granulosa; Adventitious buds; Rapid propagation; Tissue culture;
【Key words】 Tibouchina granulosa; Adventitious buds; Rapid propagation; Tissue culture;
【基金】 广东省科技计划项目2007B020801007,广州市科技计划项目2006Z2-E0091
- 【文献出处】 广东园林 ,Guangdong Landscape Architecture , 编辑部邮箱 ,2010年05期
- 【分类号】S685.11
- 【被引频次】9
- 【下载频次】158