【摘要】 从北五味子中提取分离得到一种新的莽草酸衍生物,经一维、二维核磁共振波谱(1D NMR,HMQC,HMBC)等数据解析,鉴定为莽草酸正丁酯.并采用ZORBAX Extend C18柱,以乙腈-0.05%磷酸为流动相进行梯度洗脱,检测波长为215nm,流速1.0mL/min,建立了北五味子中莽草酸正丁酯含量的高效液相色谱分析方法.结果表明,莽草酸正丁酯在2～65μg/mL浓度范围内线性关系良好,相关系数r=0.9999,平均回收率为98.25%,相对标准偏差(RSD)为1.55%,表明本法简单准确,重复性好.更多还原

【Abstract】 A new shikimic acid derivative(1) was isolated from the ethanol extract of Schisandra chinensis(Turcz.) Baill.The structure of compound 1 was elucidated as n-butyl shikimate,on the basis of the 1D NMR,HMQC and HMBC spectroscopic techniques.A RP-HPLC method was performed in a extend C18 column with a gradient elution mobile phase of acetonitrile-0.05%H3 PO4 water solution at a flow rate of 1.0 mL/min,and the detection wavelength was 215 nm.A good linearity for n-butyl shikimate was obtained within the concentration range of 2—65μg/mL(r=0.9999) ,and the recovery was 98.25%with RSD 1.55%.The method was simple,accurate with good repeatability.更多还原