【摘要】 利用TAIL-PCR技术成功克隆了麻疯树JcFAD7基因起始密码子的上游1926bp序列。利用生物信息学手段对其序列特征和潜在的调控元件进行分析表明,该序列含有4个TATA-box和6个CAAT-box,还包含光响应元件、MYB结合位点、不同的激素反应元件以及各种胁迫响应元件,说明JcFAD7的表达可能受多种因素的调控。将启动子连接到pBI101.1载体,成功构建了驱动报告基因GUS的植物表达载体。为阐明麻疯树不饱和脂肪酸的合成代谢过程和植物生长发育的调控机理奠定了基础。更多还原

【Abstract】 The upstream sequence of start codon in JcFAD7 was cloned by using TAIL-PCR technology.The target fragment was connected with pMD18-T vector and the sequence length of 1 926 bp was obtained. In addition, the sequence characteristics and its potential regulatory elements were analyzed via bioinformatics means. There were four TATA-boxes and six CAAT-boxes in this sequence. What’s more, light responsive elements, MYB binding sites, various phytohormone responsive elements and stress responsive elements also were discovered in the promoter sequence. These suggested that the expression of JcFAD7 was regulated by multiple factors. Finally, the expression vector for driving reporter gene GUS was successfully constructed. This research had laid the foundation for clarifying the synthesis process of unsaturated fatty acids in Jatropha curcas and the regulatory mechanism of plant development.更多还原