【摘要】 斜带石斑鱼(Epinephelus coioides)为我国南方近海重要网箱养殖海水鱼品种之一,而石斑鱼苗种繁育成败对其养殖规模化有着重要影响。为此,本研究比较了3种不同水培育方式下斜带石斑鱼孵化池水质变化特征。孵化在水泥池中进行,池规格为2.5m×4.0m×1.1m。试验采用益生菌和虾片作为培水材料,益生菌总活菌浓度为1.0×108cells/mL。在孵化前1周,每天同时等量往孵化池中加入益生菌和虾片2次,至试验结束,添加时间分别为早上7:00和下午13:00。处理组1、2、3益生菌日添加量分别为每池160、320、320mL,虾片日添加量分别为每池8、8、16g。每组设2个重复。石斑鱼受精卵孵化后持续培育27d,并分别在孵化后第3、8、13、18、23、27d检测水池中pH值和溶氧、总磷、活性磷、氨氮、硝酸氮、亚硝酸氮含量。结果表明:在孵化后第3d,水体中pH值从8.06下降至7.03,之后又上升至初始值。试验期间,溶解氧水平维持在6.86~7.99mg/L之间。总磷和活性磷水平随培养时间延长而增加。在孵化后第13d,亚硝酸氮水平开始升高,并在孵化后第23d达到峰值(约0.57mg/L)。硝酸氮含量也从孵化后第13d开始上升。各处理组氨氮含量均在孵化后第18d上升至最高值。试验期间,处理组2亚硝酸氮及硝酸氮水平均低于处理组1及处理组2。因此,基于水体亚硝酸氮及硝酸氮为评价指标,斜带石斑鱼孵化池中日添加益生菌及虾片适宜浓度分别为40mL/m3和1g/m3。另外,为避免可能发生的石斑鱼鱼苗亚硝酸盐中毒,应在孵化后第23d往池中注入适当新水。更多还原

【Abstract】 This study compared and discussed response of water quality in concrete nursery ponds (W 2.5 m × L 4.0 m × H 1.1 m) applied for grouper Epinephelus coioides hatching under three different water breeding regimes (group 1, 2, 3). Pepared effective microorganisms (a total active bacterium concentration of 1.0×108 cells/mL solution (EMS) together with shrimp chip (SC) were daily added. Daily addition levels of EMS for group 1, group 2 and group 3 were respectively 160 mL, 320 mL and 320 mL per pond. For SC, daily addition levels were respectively 8 g, 8 g and 16 g per pond. The experiment continued 27 days. Water quality was measured during the whole experimental period. pH values decreased from 8.06 to 7.45 on 3 days after hatching (DAH), and then increased to the initial level. Dissolved oxygen (DO) levels varied at 6.86~7.99 mg/L. Total phosphate (TP) had an increasing tendency with larvae cluture time, and similar results were observed in for Soluble reactive phosphate (SRP). On 13th, NO2-N values rapidly rose and reached a peak (about 0.57 mg/L) on 23th. NO3-N values started to increase on 13th. NH3-N values in all groups had a peak on 18th. Overall, daily additions of EMS and SC at concentrations of 40 mL/m3 and 1 g/m3 would be optimal for water breeding of grouper nursery ponds, and new seawater should be exchanged into nursery ponds on 13th.更多还原