【摘要】 将亚麻与红麻的愈伤组织在水解酶的作用下分离出原生质体,然后利用PEG法使这2种原生质体融合。结果表明,用2%纤维素酶+1%果胶酶+0.55mol/L甘露醇处理材料,酶解6h后,可以获得大量游离的、圆球形的原生质体;利用40%PEG6000及0.3mol/LCaCl2溶液(含9%甘露醇,pH9.5)将这2种原生质体融合,可以获得稳定的杂合细胞。更多还原

【Abstract】 With the method of enzymolysis, the protoplasts were released from the callus of Linum usitatissimum L. and Hibiscus cannabius L., respectively, and then they were fused by PEG as fusogen. The results showed that spherical protoplasts were available in sufficient quantities by using 2% cellulase + 1% pectinase + 0.5 mol/L mannitol to treat the callus for 6 h. Furthermore, the two different protoplasts could be fused into heterozygous protoplasts using 40% PEG fusion liquid and 0.3 mol/LCaCl2 solution (containing 9%mannitol, pH value 9.5), and these heterozygous protoplasts were stable.更多还原