【摘要】 本研究通过3年4环境的田间试验,在BIL(backcross inbred lines)群体中选取断裂比强度最大的材料NMGA-062(32.5cN/tex)和断裂比强度最小的材料NMGA-144(26.67cN/tex),利用Affymetrix棉花基因芯片,比较分析NMGA-062和NMGA-144纤维发育10d时的基因表达谱。在24029条转录本中,两个基因型中差异表达的转录本6504条,占筛选转录本总数的27.07%;其中差异表达倍数在2倍或2倍以上的转录本有3461条,占筛选转录本总数的14.41%,对其进行功能分类。对8个差异表达明显的基因(Ghi.10655.1.S1_s_at,ACO1,ARF1,SAHH,TUA6,TUA7,β-tub1,β-tub10)进行实时荧光定量PCR的检测,发现表达模式均与芯片结果一致,说明芯片数据具有生物学意义上的可重复性,结果是可信的。本研究为以后克隆及功能验证纤维发育相关基因提供了重要参考。更多还原

【Abstract】 In this research,we selected two extremely cultivars in backcross inbred lines through 3-year field trial in 4 environment and variance analysis,NMGA-062 has the best breaking tenacity of 32.5 cN/tex and NMGA144 has the lowest breaking tenacity of 26.67 cN/tex.Then,compared the gene expression profiling of NMGA-062 and NMGA-144 at 10 d by using Affymetrix microarrays.In the 24 029 transcripts,6 504 transcripts showed significant differential expression(DE) and 3461 transcripts showed 2-fold or higher levels of expression changes,occupied the total transcripts 27.07% and 14.41%,respectively.Classification of gene function was done of the transcripts which showed 2-fold or higher levels of expression changes.Through fluorescent quantitation RT-PCR analyses on different plant tissues and developing fibers of 10 d,of 8 selected DE genes including Ghi.10655.1.S1_s_at,ACO1,ARF1,SAHH,TUA6,TUA7,β-tub1,β-tub10,we found that all had similar results to the microarray analysis.The results indicated that the comparative microarray results were biologically reproducible,and the results were bilievable.It provided important reference for further studies in cloning and identifying genes responsible for fiber-related genes.更多还原