【摘要】 建立了同时检测苹果中多菌灵、噻菌灵和甲基托布津残留量的高效液相色谱(HPLC)分析方法.样品经乙酸乙酯提取,旋转蒸发仪浓缩,氮气吹干甲醇定容后,采用配有二极管阵列检测器(DAD)的HPLC测定,外标法定量.在添加不同浓度的标准品时,多菌灵、噻菌灵和甲基托布津的添加回收率分别为87.7%～118.7%、72.8%～80.3%、64.0%～66.8%.方法对多菌灵、噻菌灵和甲基托布津3种农药的检出限较低,分别为0.134、0.230和0.250mg/L,可以满足苹果汁中多菌灵、噻菌灵和甲基托布津的残留限量检测要求.检测果皮样品中的农药残留量,多菌灵的残留量为7.24×10-2mg/kg,噻菌灵和甲基托布津未检出,低于国标中规定的残留限量标准.更多还原

【Abstract】 A high performance liquid chromatographic(HPLC) method has been developed to determine the residues of carbendazim, thiabendazole and thiophanate methyl in apple. Samples were extracted with ethyl acetate, concentrated in a rotary evaporator, purged by nitrogen, added methanol to a constant volume and determined by HPLC with a diode array detector (DAD). The average spiked recoveries of carbendazim, thiabendazole and thiophanate methyl ranged from 87.7% to 118.7% , 72.8% to 80.3% and 64.0% to 66.8% at different concentrations, and the detection limits of carbendazim, thiabendazole and methyl thiophanate in apple samples were 0.134 mg/L, 0.230 mg/l, and 0.250 mg/L, respectively. The results showed the method is suitable for the determination of carbendazim, thiabendazole and methyl thiophanate in apple, and the residue of carbendazim in apple peel was 7.24×10-2 mg/kg, thiabendazole and methyl thiophanate were not detected .These concentrations were lower than the national residual limit standards for carbendazim, thiabendazole and methyl thiophanate in apple.更多还原