【摘要】 采用0.26×10-4W/cm2的紫外线(UVB)照射豚鼠背部皮肤,于照射后给予不同的融合蛋白(PTD-SOD1和PTD-SOD2)霜剂和野生型SOD霜剂涂抹受照部位皮肤,利用数码相机拍摄豚鼠背部皮肤颜色的变化情况,用Photoshop软件通过测量分析皮肤红斑度的变化来反映辐射损伤的程度,以探讨两种融合蛋白和野生型SOD对受照豚鼠的皮肤保护作用。结果表明,照射后立即给予PTD-SOD有较好的保护作用,其效果随给药剂量的增加而增加。PTD-SOD1蛋白对一次性UVB辐射损伤的保护作用最好,野生型SOD蛋白对一次性UVB辐射损伤也有保护作用,其效果与PTD-SOD2蛋白相当。更多还原

【Abstract】 The oxidized damage models of the skins on the back of guinea pigs caused by ultraviolet radiation(0.26×10-4 W/cm2)were established.Recombinant protein PTD-SOD1,PTD-SOD2 and wild-type SOD were administered on the damaged skins of guinea pigs after irradiation.The color change of the skins was recorded with a digital camera and analyzed with Photoshop software.The degree of erythema was evaluated by measuring the a value which indicated the amount of redness in the skin.An increment of a values indicates the development of erythema.It has been proved that PTD-SOD proteins are effective in protection of cutaneous damage caused by UVB radiation when the guinea pigs are administered immediately after irradiation and the protective effect is improved with the increasing of administration dose.Both PTD-SOD proteins and wild-type SOD protein were proved to have protective effect on UVB-induced oxidative damage.PTD-SOD1 was the best protective agent among three types of proteins and the other two proteins had approximately equal protective effect.更多还原