【摘要】 从湖北某纺织厂废水生化处理池中分离得到1株好氧反硝化细菌HS-N2,并对其反硝化能力进行了研究。结果表明,HS-N2在能有效去除培养基中的硝酸盐氮。在好氧条件下,30℃培养24h,能将10mmol/L硝酸盐氮去除95%以上;在兼性厌氧条件下,24h的脱氮率达到85%。研究了该菌株的好氧反硝化特性。结果表明:其反硝化最适温度和pH分别为30℃和7.0。设计特异性引物,扩增HS-N2菌株的16SrDNA并测序,运用Blast比对构建了进化树,发现该菌与肠炎沙门氏菌(Salmonella enteritidis)的同源性高达100%。结合形态观察和生理生化鉴定,初步鉴定该菌属于沙门氏菌属(Salmonella),命名为Salmonella sp.HS-N2。目前关于沙门氏菌属具有反硝化能力的菌株鲜有报道。更多还原

【Abstract】 A bacterial strain HS-N2 was isolated from a textile mill in Hubei Province.Results showed that HS-N2 was capable of denitrification under aerobic condition and facultative anaerobic condition.Nitrate of 10mmol/L could be efficiently removed by strain HS-N2 and the nitrogen removal rate was above 95% at 30℃ for 24h culture.Factors that affecting anaerobic denitrification by strain HS-N2 were determined.Results indicated that the most optimum temperature and pH value for aerobic denitrification were at 30℃ and 7.0 respectively.According to morphological characteristics,physiological biochemical test and sequence analysis of 16S rDNA,strain HS-N2 was identified as Salmonella Lignieres.更多还原