【摘要】 为了探索蝴蝶兰中MADS盒基因在花发育过程中对花器官发育的调控机制,目前从蝴蝶兰中克隆出一个MADS盒基因家族B族基因PhAP3,并对其进行了序列分析和基因表达研究.聚类分析结果表明PhAP3是拟南芥AP3基因的同源基因,但基因表达分析表明PhAP3可以在植物的营养器官和生殖器官中均有表达,这与典型的AP3类基因有所不同.更多还原

【Abstract】 To explore the mechanism of MADS box genes in controlling the development of floral organs identity in Phalaenopsis,a novel B-class gene,PhAP3,from Phalaenopsis orchid was cloned.Phylogenetic analysis and expression characterization revealed that it is homolog to the AP3 gene of Arabidopsis.The results of RT-PCR demonstrated PhAP3 was expressed in both the vegetative and reproductive organs of Phalaenopsis,unlike typical AP3-like genes.更多还原