节点文献

武汉市霍乱弧菌中毒力相关基因及分类鉴定

Detection of virulence related genes and molecular typing by PCR in Vibrio Cholerae from Wuhan City

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 江元山熊燕陈智龙一兵朱焰周军波

【Author】 JIANG Yuan-shan,XIONG Yan,CHEN Zhi,LONG Yi-bing,ZHU Yan,ZHOU Jun-bo. Wuhan Centers for Disease and Prevention Control,Wuhan 430015,China

【机构】 武汉市疾病预防控制中心

【摘要】 目的为了解武汉地区历年收集的霍乱弧菌毒力相关基因分布情况,并对这些菌株进行分类鉴定。方法分别对霍乱弧菌中的ctxA、zot、ace、ompU、tcpA、toxR、tcpI与hlyA等基因进行PCR检测,并对所有霍乱弧菌进行PCR分类鉴定。结果分离的大部分霍乱弧菌菌株携带有所有的毒力基因,有3株细菌仅有个别非核心毒力基因扩增阳性;该PCR方法分类对O1型所有菌株都能很好地进行分类鉴别,对O139型则与血清型鉴定存在不一致情况。结论PCR方法对产毒型和非产毒型O1/O139霍乱弧菌以及O1/O139和非O1/O139霍乱弧菌鉴定效率非常好。

【Abstract】 Objective To detect and analyze the distribution of virulence related genes and typing by PCR assay in Vibrio cholerae isolated from Wuhan city.Methods 9 pair probes of PCR assay for the detection of the virulence related genes including ctxA、zot、ace、ompU、tcpA、toxR、tcpI and hlyA in Vibrio cholerae were detected,and molecular typing was performed.Results Most of the Vibrio cholerae isolates carried all of the virulence related genes,and only few minor virulence related genes were detected in three isolates.The PCR assays were in accordance with serotypes in O1 strains,and only few of PCR typing assays were inconsistent with serotypes in O139 strains.Conclusions The study showed that PCR assays could be used in detecting virulence related genes and molecular typing in Vibrio cholerae isolates.

  • 【文献出处】 公共卫生与预防医学 ,Journal of Public Health and Preventive Medicine , 编辑部邮箱 ,2010年01期
  • 【分类号】R450;R446.5
  • 【被引频次】3
  • 【下载频次】111
节点文献中: 

本文链接的文献网络图示:

本文的引文网络