【摘要】 背景与目的雷氏大疣蛛蜘蛛毒素作为新药有可能用于癌症的治疗,本研究旨在探讨雷氏大疣蛛蜘蛛毒素对人肺癌细胞A549的作用及机理。方法应用MTT法检测雷氏大疣蛛蜘蛛毒素对人肺癌细胞A549增殖的影响,比色法检测过氧化氢酶活性,改良的硫代巴比妥酸荧光法检测丙二醛含量;流式细胞仪检测细胞凋亡率。采用免疫印迹分析A549细胞中P38MAPK蛋白的表达。结果雷氏大疣蛛蜘蛛毒素可抑制A549细胞增殖,使CAT活性和MDA的形成增加,且使P38MAPK的表达较对照组明显增多。结论雷氏大疣蛛蜘蛛毒素抑制A549细胞增殖可能与CAT活性和MDA的形成增加以及P38MAPK的表达降低相关。更多还原

【Abstract】 Background and objective The spider venom may inspire new drugs to treat cancer. The aim of this study is to investigate the effects and mechanisms of spider venom on lung adenocarcinoma cell A549. Methods The proliferation of lung adenocarcinoma A549 cells was detected by MTT. The apoptosis rate was observed with MTT assay and flow cytometer. The activity of catalase was detected by colorimetry. The malondialdehyde (MDA) content was determined by improved thiobarbituric acid fluorometric method. The expression of P38MAPK protein was analyzed with Western blot. Results Spider venom can remarkably inhibite the proliferation of lung adenocarcinoma A549 cells, increased activity of catalase and MDA content, down-regulated expression of P38MAPK compared with the control group. Conclusion The reduced proliferation of lung adenocarcinoma A549 cells by spider venom is may be associated with the increased of activity of catalase and MDA content and decreased expression of P38MAPK.更多还原