【摘要】 背景与目的核苷酸切除修复交叉互补(excision repair cross-complementating,ERCC)基因家族能对核苷酸进行切除和修复以减少DNA的损伤。如果损伤性的核苷酸切除修复机制受损,可增加基因组的不稳定性,进而导致肿瘤发生更多的恶性表型行为。因此本研究旨在评价肿瘤ERCC1的表达对早期非小细胞肺癌(non-small cell lung cancer,NSCLC)手术切除后患者的生存率的影响。方法采用免疫组化方法检测ERCC1在肿瘤中的表达,以评估ERCC1表达对118例接受过根治性切除的I期NSCLC患者生存率的意义。结果肿瘤ERCC1表达阳性与阴性的患者3年生存率分别为86.12%、65.46%,两者差异具有统计学意义(P=0.025)。多变量分析表明,ERCC1高表达对较长生存期具有独立预测性。结论手术切除的NSCLC患者中,ERCC1阳性表达者的生存率较阴性表达者高。可见,完整的DNA修复机制可减少导致肿瘤恶性潜能的基因畸变的积累,从而减少复发的危险。更多还原

【Abstract】 Background and objective Proteins of the nucleotide excision repair pathway can repair DNA damage. The excision repair cross-complementing (ERCC) gene family reduce damagement of DNA by nucleotide excision and repair. The aim of this study is to investigate the expressions of ERCC1 (members of DNA repair gene family) in patients with non-small cell lung cancer (NSCLC) as well as their clinical prognostic significance. Methods Expression levels of ERCC1 were detected by IHC in 118 stage I NSCLC patients. Kaplan-Meier survival curve, and Cox multivariate regression analysis were used for statistical analysis. Results The patients with high expression of ERCC1 had significantly longer survival time than those with low expression of ERCC1, and Cox multivariate regression analysis showed that expression of RRM1 was an independent prognostic factor for NSCLC patients. Conclusion NSCLC patients with high ERCC1 expression have a better survival when compared to patients with low ERCC1 expression. Therefore, an intact DNA repair mechanism may reduce the accumulation of genetic aberrations that are thought to contribute to a tumor malignant potential and therefore the risk of relapse after definitive treatment.更多还原