【摘要】 【目的】研究荷泽口服液对生长期高脂诱导肥胖大鼠血甘油三脂、体脂及脂肪细胞大小的影响,并对其影响机制进行初步探讨。【方法】将实验大鼠随机分为正常组、过重组和中药组,分别饲以基础饲料、高脂饲料并灌胃生理盐水、和高脂饲料并灌胃中药,至第6周末测三组大鼠血清甘油三酯水平、腹膜后、附睾脂肪组织含量,腹膜后脂肪组织脂肪细胞直径;RT-PCR测肝组织乙酰辅酶A羧化酶表达水平。【结果】过重组大鼠体重、甘油三酯水平、脂肪组织含量、脂肪细胞直径、乙酰辅酶A羧化酶基因表达显著高于正常组(P均<0.05);中药组大鼠体重与过重组比较差异无统计学意义(P>0.05),但是其甘油三酯水平、脂肪组织含量、脂肪细胞直径、乙酰辅酶A羧化酶基因表达显著低于过重组(P均<0.05)。【结论】荷泽口服液具有减肥降脂作用,肝脏脂肪酸合成减少可能是其减肥降脂作用的途径之一。更多还原

【Abstract】 【Objective】To investigate the effect of chinese herbs on triglyceride,body fat masses,and on gene expression of acetyl-CoA carboxylase in liver of high-fat diet induced obese rats. 【Methods】30 male Wistar rats were randomly divided into three groups:a normal group(n=10),fed with normal diet;an obese control group(n=10),fed with high fat diet and with a controlled 0.9% saline;and a chinese herbs group(n=10),fed with high fat diet and with a chinese medicine.After 6 weeks,the rats of the obese control group and the chinese herbs group were much heavier than that of the normal group.Then,blood samples were drawed by heart puncture and concentrations of triglyceride(TG) were measured in all three groups.After the rats were killed,the retroperitoneal and epididymal fat masses were carefully collected and weighed and diameters of adipocytes were measured.Livers were collected and mRNA levels of acetyl-CoA carboxylase(ACC) were evaluated by RT-PCR. 【Results】Body weight gain,serum TG,body fat masses and ACC mRNA were higher in obese control group than those in normal group(P<0.05).As compared with obese control group,chinese herbs significantly reduce weight gain,body fat masses,serum TG and ACCmRNA(P<0.05). 【Conclusion】Altering hepatic gene of ACC by chinese herbs plays a potential role in down-regulation of adiposity.更多还原