【摘要】 目的:探讨白细胞介素15(IL-15)对骨髓增生异常综合征(MDS)造血细胞caspase-3mRNA表达的影响,以探讨IL-15抑制MDSCD34+造血干/祖细胞凋亡的可能机制。方法:采用吸附单克隆抗体的免疫磁珠分离系统分离纯化18例MDS患者骨髓CD34+细胞,采用逆转录-聚合酶链式反应(RT-PCR)检测caspase-3mRNA表达水平,观察IL-15对它们的影响。结果:发现IL-15下调体外培养的MDS造血前体细胞cpp32基因mRNA的表达在0～100ng/mL范围内呈剂量依赖性,其差异具有统计学意义;从d1～d8,随着IL-15作用时间延长,下调caspase-3基因mRNA表达的能力越强,其差异具有统计学意义。结论:IL-15可呈时间与剂量依赖性的下调体外培养的MDS造血前体细胞caspase-3基因mRNA的表达。IL-15下调MDSCD34+造血干/祖细胞caspase-3mRNA的表达,可能为其抑制该细胞凋亡的作用机制之一。更多还原

【Abstract】 Objective:To investigate the effects of interleukin-15(IL-15)on caspase-3 mRNA expression of hematopoietic cells in myelodysplastic syndromes(MDS)patients.Methods:CD34+cells in bone marrow from 18 MDS patients were purified with an immunomagnetic beads sorting system.RT-PCR was used to detect the expressing level of caspase-3 mRNA,and the effect of IL-15 on them was observed in order to discuss the possible mechanism of IL-15 in suppressing apoptosis of hematopoietic precursors.Results:IL-15 could put down caspase-3 mRNA level of hematopoietic cells in myelodysplastic syndromes(MDS)in a dose and time-dependent manner.The difference had statistical significance.Conclusions:Decreasing the expressing level of caspase-3 mRNA could be one of the mechanism of IL-15 in suppressing apoptosis of hematopoietic precursors.更多还原