【摘要】 为探讨鸡胚致死率在检验鸡大肠埃希菌致病力方面的应用潜力,试验采用SPF级12日龄鸡胚和1日龄公雏鸡分别接种鸡大肠埃希菌菌株,检测20株鸡大肠埃希菌菌株对12日龄鸡胚和1日龄公雏鸡的致死率的相关性。结果显示,8株来源于患败血症鸡只的菌株致病力比较高,鸡胚致死率达到40%~70%;而来源于粪便的12株菌株中,10株菌株的致病力较低,其鸡胚致死率在0%~20%之间;雏鸡致死率与鸡胚致死率是相关的。表明鸡胚致死试验可反映出大肠埃希菌的毒力强弱,即鸡胚致死率可作为检验鸡大肠埃希菌致病力强弱的一种方法。更多还原

【Abstract】 In order to determine the potential of ELA(embryo lethality assay) for determining virulence of avian E.coli,an embryo lethality assay of 20 isolates was linked with a chicken ethality assay to analyze their association.In this study,8 isolates from colisepticemia were higher virulent,with 40%-70% embryo lethality.10 of 12 from chicken feces were less virulent,with 0%-20% embryo lethality.And the embryo lethality was associated with chicken lethality.This study suggested that ELA may be correlated with virulence of avian E.coli,and may be a method for determining virulence of avian E.coli.更多还原