【摘要】 目的观察1,25(OH)2D3对实验性自身免疫性甲状腺炎(EAT)大鼠炎症及Th1/Th2细胞的干预效果。方法 48只雌性Wistar大鼠随机分为预防组(n=10),治疗组(n=10),阳性对照组(n=12)和阴性对照组(n=16)。除阴性对照组外,其余大鼠均给予pTG免疫致敏。预防和治疗组每只大鼠使用维生素D35μg/kg.,隔日腹腔注射,分别从建模0～6周和2～8周作干预,阴性对照组和阳性对照组应用花生油作对照。各组大鼠在处死后取甲状腺组织做病理学检查,检测血清自身抗体,及细胞因子的改变。结果阴性对照大鼠的甲状腺结构完整;阳性对照组大鼠甲状腺滤泡结构破坏严重,部分滤泡萎缩或消失,1,25(OH)2D3干预组大鼠甲状腺结构保持较完整,萎缩滤泡减少,淋巴细胞浸润不明显。与阴性对照组相比,预防组其自身抗体及细胞因子水平均无显著性差异;治疗组大鼠血清抗体水平与同时期的阳性对照相比水平明显降低(P<0.05),但较阴性对照明显升高(P<0.05);与阳性对照组相比,治疗组大鼠IFN-γ及IL-12水平显著下降(P<0.05),IL-4和IL-10水平升高(P<0.01)。结论在EAT大鼠建模开始就使用1,25(OH)2D3可使大鼠甲状腺组织保持较完整形态,维持血清抗体及细胞因子正常范围。对EAT大鼠,1,25(OH)2D3有可能减缓病理损害,调整细胞因子的失衡。更多还原

【Abstract】 Objective To observe the effect of 1,25 (OH)2D3 on thyroid inflammation and Th1/Th2 cells in rats with experimental autoimmune thyroidits (EAT). Methods Forty-eight female Wistar rats were randomly divided into 4 groups, namely the prevention group treated with 1, 25-(OH)2D3 from 0 to the 6th week (n=10), treatment group with 1,25(OH)2D3 treatment from the 2nd to the 8th week (n=10) after immune sensitization, positive control group (n=12) and the negative control group ( n=16). All the rats were challenged with porcine thyroglobulin for immune sensitization until the 6th or 8th week except for those in the negative control group. In the prevention group and treatment group, the rats received 1,25(OH)2D3 at 5 μg/kg by intraperitoneal injection every other day, while those in the positive and negative control groups were given peanut oil instead. The thyroid pathologies, serum autoantibody level and cytokine levels were examined after the treatments. Results The thyroid gland remained structurally intact in the negative control group. In the positive control group, the thyroid showed obvious inflammatory change with structural disruption and even disappearance of the thyroid follicle. The structure of the thyroid gland follices was intact in the prevention group and treatment group. No significant differences were found in the autoantibody and cytokine levels between the prevention group and negative control group (P>0.05). Compared with the positive control groups, the autoantibody and IFN-γ and IL-12 levels decreased significantly in the treatment group, but the levels of IL-4 and IL-10 were markedly increased (P<0.05). Conclusion 1,25 (OH)2D3 given before the establishment of the EAT model helps maintain structural integrity of the thyroid gland and normal levels of the antibodies and cytokines in rats. 1,25(OH)2D3can ameliorate the pathologicalchangesofthe thyroid gland and correctthe cytokine disequilibriumin ratswith EAT.更多还原