【摘要】 目的通过观察六味地黄丸对自发性2型糖尿病OLETF大鼠胰腺形态学的影响,探讨六味地黄丸预防糖尿病的可能机制。方法自发性2型糖尿病大鼠OLETF鼠40只,随机分为六味地黄丸干预组和对照组,LETO鼠10只作为正常对照组。干预组从8周龄起以六味地黄丸2.4 mg.kg-1.d-1灌胃给药,其余两组以等量清水灌胃。定期OGTT试验。于8、32和40周龄时分批宰杀大鼠。分离胰腺。HE染色观察胰岛结构;Masson三色染色观察胰岛纤维化程度。结果随着周龄的延长,对照组大鼠胰岛逐渐出现纤维化,萎缩;干预组的胰岛结构保存明显好于对照组。通过Masson染色,可见干预组胰岛纤维化程度明显轻于同周龄对照组。LETO组大鼠胰腺结构随着周龄延长无明显变化。干预组从30周龄开始出现糖尿病,至40周糖尿病发病率28.6%,显著低于对照组(P<0.01)。结论六味地黄丸早期干预能明显保护OLETF大鼠胰岛结构减少其纤维化,可能是其预防糖尿病的机制之一。更多还原

【Abstract】 Objective To study the effects of Liuweidihuang pills on pancreatic islet structure in OLETF(Otsuka Long-Evans Tokushima Fatty) rats.Methods Forty male OLETF rats were divided randomly into Liuweidihuang pills group(Liuwei group) and control group(n=20).Ten male LETO rats were used as normal control group(LETO group).The rats in Liuwei group were given Liuweidihuang pills at the daily dose of 2.4 mg/kg intragastrically since the age of 8 weeks.Blood glucose was determined by OGTT.The rats were sacrificed at 8,32 and 40 weeks and the pancreatic tissue was isolated to examine the morphological changes of the pancreas by HE staining and Masson trichrome staining.Results As the age of the rats increased,the pancreatic islets in the control group gradually showed fibrosis and islet atrophy,which were not found in Liuwei group.Masson staining visualized no fibrosis in Liuwei group.No significant pathological change occurred in the pancreatic islet of LETO rats.The rats in Liuwei group developed diabetes since 30 weeks of age and the incidence was 28.6% at 40 weeks,significantly lower than that in the control group(P<0.01).Conclusion Liuweidihuang pills can prevent degeneration of the pancreatic islets in spontaneous OLETF rats.更多还原