【摘要】 目的研究Hedgehog通路特异性抑制剂cyclopamine联合喜树碱对口腔肿瘤细胞增殖、凋亡的影响。方法 CCK8法检测cyclopamine联合喜树碱对HSQ-89细胞增殖的影响;流式细胞仪检测cyclopamine联合喜树碱对HSQ-89细胞凋亡的影响;RT-PCR检测cyclopamine及喜树碱处理后HSQ-89细胞凋亡相关分子表达变化情况。结果 Cyclopamine联合喜树碱较单用喜树碱能更加明显抑制HSQ-89细胞活性。Cyclopamine处理后HSQ-89细胞中Bcl-2表达下降,而Bcl-xl、Bid等表达不变,联合喜树碱后Bcl-2表达进一步减少。结论 Cyclopamine联合喜树碱能有效抑制Bcl-2表达而发挥抑制癌细胞增殖,并诱导凋亡效应。更多还原

【Abstract】 Objective To study the mechanism underlying the effect of combined use of cyclonpamine and hydroxycamptothecin in inducing the apoptosis of human oral squamous cell carcinoma cell line (OSCC) HSQ-89. Methods CCK8 assay was used to investigate the inhibitory effect of cyclopamine on HSQ-89 cells. Flow cytometry (FCM) was employed to examine the cell apoptosis following combined treatment with cyclonpamine and hydroxycamptothecin. Reverse transcription polymerase chain reaction (RT-PCR) was applied to detect the mRNA expressions of Bcl-2, Bcl-xl, and Bid in HSQ-89 cells after the treatments. Results Combined treatment with cyclonpamine and hydroxycamptothecin significantly inhibited the cell proliferation compared with hydroxycamptothecin treatment alone, also resulting in a significantly higher apoptosis rate of the cells (P<0.05). The mRNA level of Bcl-2 was significantly decreased after the treatments, especially after the combined treatment. Cyclopamine produced no significant effect on the mRNA levels of Bcl-xl and Bid in the cells. Conclusion The combined use of cyclopamine and hydroxycamptothecin significantly down-regulates the expression on of bcl-2 to induce the apoptosis of human OSCC cell line HSQ-89.更多还原