【摘要】 目的探讨组胺H1受体对新生大鼠延髓脑片吸气神经元放电的调制作用。方法制作新生大鼠离体延髓脑片标本,保留舌下神经根,使用改良Kreb’s液(MKS)灌流延髓脑片。用吸附电极记录到舌下神经根呼吸相关节律性放电活动(RRDA)后,以细胞外记录方式在面神经后核内侧区同步记录吸气神经元放电活动。稳定记录吸气神经元放电20min后,用5μmol/L组胺灌流延髓脑片20min;使用空白MKS冲洗至RRDA和吸气神经元放电基本恢复后;再用含组胺H1受体特异拮抗剂10μmol/Lpyrilamine的MKS灌流脑片。观察组胺和pyrilamine对吸气神经元放电的呼吸周期(RC)、吸气时程(TI)、呼气时程(TE)、放电积分幅度(IA)和单位放电的峰频率(PF)等指标的作用。结果给予组胺后,吸气神经元放电的RC缩短25.86%、TE缩短27.03%,TI、IA和PF无变化;使用pyrilamine后RC延长21.46%、TE延长22.15%,TI,IA和PF无变化。结论组胺H1受体参与调节新生大鼠离体延髓脑片吸气神经元放电活动。激活H1受体对吸气神经元放电有兴奋作用。更多还原

【Abstract】 Objective To investigate the role of histamine H1 receptors in modulating the discharge activities of the inspiratory neurons in the nucleus retrofacialis of neonatal rats. Methods Brainstem slices were obtained from neonatal rats containing the medial region of the nucleus retrofacialis (mNRF) with the hypoglossal nerve (XII nerve) rootlets retained. The rhythmic discharges of the inspiratory neurons (I neurons) and activities of the XII nerve rootlets were simultaneously recorded using microelectrodes and suction electrodes, respectively. The role of H1 receptors in modulation of the discharge activities of the inspiratory neurons was investigated using the H1 receptor agonist histamine and its specific antagonist pyrilamine dissolved in modified Kreb’s solution for slice perfusion. Results Histamine shortened the respiratory cycle (RC) and expiratory time (TE) of the neurons in the brain slices, and pyrilamine produced the opposite effects. Neither histamine nor pyrilamine affected the inspiratory time (TI), integral amplitude (IA) or the peak discharge frequency (PF) of the I neurons. Conclusion H1 receptors play an excitatory role in the modulation of the discharge activities of the inspiratory neurons in neonatal rat brainstem slices.更多还原