【摘要】 目的制备可注射骨修复中国青山羊胫骨腔穴性骨缺损模型。方法于青山羊双侧胫骨内侧平台下制作直径1.2cm的腔穴性骨缺损,术后4、8周,通过X片观察、硬组织切片形组织学观察,图像分析新评价青山羊胫骨腔穴性缺损动物模型的可靠性。结果术后4、8周实验组X片观察、组织学检查均显示中国青山羊胫骨腔穴性骨缺损部无明显骨组织形成,图像分析结果示骨缺损区域成骨面积比例分别为(8.79±3.63)%、(15.41±4.21)%。结论中国青山羊胫骨腔穴性骨缺损模型满足了可注射骨在四肢骨缺损修复中的应用要求。模型制作简单、可靠性强、可注射骨应用时方便、成骨局部环境与力学环境适合于临床四肢骨缺损修复研究需求。更多还原

【Abstract】 Objective To prepare a goat model of tibial bone hole defect suitable for studies of bone defect repair using tissue-engineered injectable bone materials. Methods A circular hole bone defect 1.2 cm in diameter was induced below the tibial medial plateau of the goat. X-ray, histological inspection, and image analysis were carried out to evaluate the validity of the model in simulating limb bone defect for the study of tissue-engineered injectable bone materials. Results At 4 and 8 weeks after the operation, neither X-ray nor histological examination showed obvious bone tissues in the bone defect. Image analysis showed a area of new bone tissue formation of (8.79±3.63)% in the total defect area at 4 weeks, which increased to (15.41±4.21)% at 8 weeks. Conclusion The goat model of tibial bone hole defect established in this study is suitable for studying the ability of injectable bone materials for repairing limb bone defect, and offers a simple and reliable means to simulate the local condition of bone regeneration and mechanical environment of bone defect in the limbs.更多还原