【摘要】 以低光强生态型盾叶薯蓣(Dioscorea zingiberensis)为材料,研究强光高温(32℃)对盾叶薯蓣量子产额和77K荧光光谱的影响,以探讨强光高温胁迫与光合作用的关系。结果表明:短期高温强光胁迫引起最大量子产额显著下降,但能迅速恢复,且存在超补偿现象。强光胁迫首先破坏了光系统Ⅱ,表现为胁迫后在77K荧光光谱中出现了F680的荧光峰。670 nm红光诱导后引起部分捕光系统Ⅱ的色素蛋白转移至光系统Ⅰ,导致光系统Ⅰ的荧光发射量增加;716 nm红光诱导后引起捕光系统Ⅱ部分色素蛋白转移到光系统Ⅱ,导致光系统Ⅱ的荧光发射量增加。强光高温胁迫后各个荧光峰的差异程度减少。研究发现,强光高温胁迫引起光系统的能量重新调整,从而出现状态转变;依据F672和F675两个荧光峰的首次出现,预测叶绿体类囊体膜上含叶绿素的多肽或蛋白亚基至少有16个。更多还原

【Abstract】 The effects of strong light and high temperature on quantum yield and 77K fluorescence spectra of Dioscorea zingiberensis were tested.The results showed that the maximal quantum yield decreased under strong light and high temperature for short time,but it could recover quickly and existed overcompensation.Strong light firstly destroyed the photosystem Ⅱ,which caused the appearance of F680 fluorescence peak in 77K fluorescence spectra.After induced with 670 nm red light,parts of light-capture system Ⅱ transferred to photosystem Ⅰ,which resulted the increase of photosystem Ⅰ fluorescence.After induced with 716 nm red light,some pigment proteins of light-capture system Ⅱ transferred to photosystem Ⅱ,which increased the fluorescence of photosystem Ⅱ.The difference of each fluorescence peak reduced after treatment.In conclusion,the stress by strong light and high temperature causes energy re-adjusted in two photosystems and status changed from one to another.The researches have reported the appearance of F672 and F675 fluorescence peak for the first time and predicted that there are at least 16 pigment complexes on the thylakoid membrane in chloroplast.更多还原