【摘要】 建立了鲶Silurus asotus的TRAP-PCR反应体系,采用24个TRAP引物组合对鲶人工选育群体进行引物适用性的研究,将获得的多态性位点用于研究鲶群体的遗传结构。结果表明:有9个引物组合可产生有效位点,其中多态引物占所筛选引物总数的37.5%,平均每条引物扩增出15个位点。共扩增出135个位点,多态位点数为75,多态位点比例为55.56%。说明TRAP标记技术适用于鲶的遗传多样性研究,可以作为构建鲶遗传连锁图谱的分子标记。更多还原

【Abstract】 TRAP(Target Region Amplified Polymorphism) was first used for research of genetic diversity in catfish Silurus asotus.Nine pairs of primers selected from 24 primers produced good amplified patterns with 37.5% of polymorphic primer was in all amplified primers.There were 15 loci amplified by one primer,and 75 polymorphic loci were obtained in all 135 amplified loci with 55.56% of polymorphic loci.The genetic variation analyses showed that TRAP marker can be used in genetic structure research of the catfish.The higher percentage of polymorphic primer number indicated that TRAP as a DNA molecular marker can be used in genetic linkage map research for Silurus asotus.更多还原