【摘要】 目的应用核磁共振氢谱(nuclear magnetic resonance 1H,1H NMR)和主成分分析方法研究慢性乙肝患者血清的代谢组变化。方法临床正常人血清8例和慢性乙肝患者血清20例,采用BRUKER500MHz超导核磁共振波谱仪进行检测,并运用PCA分析方法,得出慢性乙肝患者血清代谢组的特征,探讨相关生化过程的改变。结果分析结果显示慢性乙肝患者和正常人血清的代谢组具有明显差异。乙肝患者血清中的脂和葡萄糖是升高的,而乳酸、丙氨酸、缬氨酸、谷氨酰胺以及胆碱类物质则是降低的,且具有统计学意义(P<0.05)。结论通过上述代谢物的变化可以区分正常人和慢性乙肝患者。这种基于核磁共振氢谱和主成分分析的代谢组学方法可以为乙肝的诊断提供可靠的分子水平上的代谢依据。更多还原

【Abstract】 Objective To characterize the metabolite composition(i.e.,metabonome) of the serum samples from chronic hepatitis B patients by using nuclear magnetic resonance 1H(1H NMR) spectroscopy combined with principal component analysis(PCA).Methods The 1H NMR sepctra were obtained for the serum samples from 8 healthy controls and 20 chronic hepatitis B patients,and the spectra data were analyzed by PCA to demonstrate the metabonomic changes.Results We found that there were evident differences in metabonomics between the healthy controls and chronic hepatitis B patients.The levels of lipids and glucose were significantly increased in the chronic hepatitis B patient and the levels of lactate,alanine,valine,glutamine and phosphocholine/choline were significantly decreased compared with the healthy controls(P<0.05).Conclusion Our results indicate that the metabonomes of serum from chronic hepatitis B patients are different from those of the healthy controls.Serum 1H NMR sepctroscopy combined with PCA may offer reliable biochemistry evidence for diagnosis of chronic hepatitis B at molecular level.更多还原