酸诱导人食管上皮细胞凋亡中P38和JNK的表达

【摘要】 目的观察酸对人正常食管上皮细胞凋亡及P38、JNK表达的影响。方法体外培养人正常食管粘膜上皮细胞,分为酸作用组(pH4.0、5.0、6.0)及正常对照组。采用流式细胞仪测定细胞凋亡。细胞免疫化学染色测定P38、JNK蛋白表达。结果与对照组相比,酸作用组的细胞凋亡率明显增加。酸作用组可见p-P38蛋白表达,无p-JNK表达。正常对照组无p-P38、p-JNK蛋白表达。结论酸可诱导人正常食管上皮细胞凋亡,与P38表达上调有关。更多还原

【Abstract】 Objective To observe the effects of acid exposure on cell apoptosis and the expression of P38 and JNK in normal human esophageal epithelial cells in vitro.Methods Normal human esophageal epithelial cells cultured in vitro were divided to acid group(pH4.0,5.0,6.0)and the control group.Cell apoptosis was assessed using flow cytometry.The expression of P38 and JNK protein were determined by cell immunochemistry. Results Acid-exposed esophageal epithelial cells exhibited a significant increase in apoptosis and the level of phosphorylated P38 protein,without the expression of phosphorylated JNK protein.There were negative expression of phosphorylated P38 and JNK protein in the control group.Conclusion Acid exposure induces apoptosis in normal human esophageal epithelial cells by activating the P38 pathway.更多还原