【摘要】 采用ISSR分子标记,对经老化处理的不同发芽率水平扁蓿豆种质进行遗传完整性分析。结果表明:用10个有效引物对4个不同发芽率居群进行PCR扩增,共检测到48个等位基因,每个位点的等位基因数为3～8个,平均为4.8个;老化处理群体的多态性位点百分率(PPB)、等位基因数(Na)、有效等位基因数(Ne)、基因多样性指数(H)、Shannon指数(I)等遗传参数,与对照群体的遗传参数相比都有所下降,表明老化处理后群体内的遗传多样性低于对照群体的遗传多样性;与对照群体相比,发芽率为20%时,各遗传参数呈极显著性差异,说明扁蓿豆种质更新发芽率临界值在20%～40%;ISSR分子标记可以作为老化扁蓿豆种子遗传完整性变化的检测方法,同时认为对于异质种质资源材料,低发芽率更新标准不利于种质资源遗传完整性的保持。更多还原

【Abstract】 The effects of artificial aging treatments on genetic integrity of Medicago ruthenica germplasm with different germination rates were studied by using ISSR markers.The results showed that 48 alleles were detected, number of alleles per locus was between 3 to 8,mean number of alleles was 4.8 with 10 primers amplification by PCR;the percentage of polymorphic bands,number of alleles(Na),effective number of alleles(Ne),Nei’s gene diversity index(H)and Shannon’s information index(I) were lower in artificially aged population than in CK population;the number of alleles in population with 20% germination rate was lower than that of CK population,and the difference was significant(P<0.01),regeneration germination was between 20% to 40%;ISSR molecular markers can be used to detect the genetic integrity of Medicago ruthenica;a low germination percentage standard for regeneration was not proper for maintaining the genetic integrity of germplasm resource.更多还原